Journal: Journal of Ovarian Research

Article Title: Notch2 improves granulosa cell functions in premature ovarian failure by activating the Wnt2/β-catenin pathway

doi: 10.1186/s13048-025-01745-9

Figure Lengend Snippet: Notch2 interacted with Wnt2 and β-catenin in POF mice. ( A ) qPCR was used to detect Wnt-2 and β-catenin mRNA expressions in the ovarian tissues. ( B ) Immunohistochemistry was applied to measure Notch2 protein expression in the ovarian tissues. Magnification×200, scale bar = 100 μm. ( C ) Western blot analysis was utilized to test Notch2 pathway-related protein expressions (Notch2, Jag1 and Hes2) and Wnt2/β-catenin pathway-related protein expressions (Wnt2, β-catenin, Axin2 and LEF1) in the ovaries. ( D ) Co-immunoprecipitation (Co-IP) was conducted to further verify the protein-protein interactions between Notch2 and Wnt2. P < 0.05 and P < 0.01 vs. Control; # P < 0.05 and ## P < 0.01 vs. oe-NC; @ P < 0.05 and @@ P < 0.01 vs. oe-Notch2. Results were presented as mean ± SD

Article Snippet: Jag1 , BIOSS , bs-1448R , 1:1000.

Techniques: Immunohistochemistry, Expressing, Western Blot, Immunoprecipitation, Co-Immunoprecipitation Assay, Protein-Protein interactions, Control

Journal: Journal of Ovarian Research

Article Title: Notch2 improves granulosa cell functions in premature ovarian failure by activating the Wnt2/β-catenin pathway

doi: 10.1186/s13048-025-01745-9

Figure Lengend Snippet: Notch2 acted on the Wnt2/β-catenin pathway in POF cells. ( A ) qPCR analysis for the expressions Wnt2 and β-catenin mRNAs in KNG cells. ( B ) Western blot analysis for the expressions of Notch2, Jag1, Hes2 Wnt2, β-catenin, Axin2 and LEF1 proteins in KNG cells. P < 0.05 and P < 0.01 vs. Control; * P < 0.05 and ** P < 0.01 vs. Model; # P < 0.05 and ## P < 0.01 vs. sh-NC; @ P < 0.05 and @@ P < 0.01 vs. sh-Notch2. Results were presented as mean ± SD

Article Snippet: Jag1 , BIOSS , bs-1448R , 1:1000.

Techniques: Western Blot, Control

Journal: Journal of Ovarian Research

Article Title: Notch2 improves granulosa cell functions in premature ovarian failure by activating the Wnt2/β-catenin pathway

doi: 10.1186/s13048-025-01745-9

Figure Lengend Snippet: βcatenin knockdown inhibited Wnt2/β-catenin pathway in POF cells treated with sh-Notch2 and SKL2001 ( A ) qPCR analysis for the expressions Wnt2 and β-catenin mRNAs in KNG cells. ( B ) Western blot analysis for the expressions of Notch2, Jag1, Hes2 Wnt2, β-catenin, Axin2 and LEF1 proteins in KNG cells. # P < 0.05 and ## P < 0.01 vs. sh-Notch2 + SKL2001 + sh-NC. Results were presented as mean ± SD

Article Snippet: Jag1 , BIOSS , bs-1448R , 1:1000.

Techniques: Knockdown, Western Blot

Journal: Biomedical Reports

Article Title: Notch signaling pathway regulates the progression of fetal growth restriction through mediating immune dysfunction

doi: 10.3892/br.2025.1989

Figure Lengend Snippet: The expression and distribution of Notch1 and Jagged1 is decreased in PBMCs and placenta. (A-E) The expression of Notch1 and Jagged1 in PBMCs and placenta was detected by (A and B) reverse transcription-quantitative PCR, (C) western blotting, (D) immunofluorescence and (E) immunohistochemistry, respectively. ** P<0.01 and *** P<0.001 vs. control. Scale bar, 100 µm. PBMCs, peripheral blood mononuclear cells; FGR, fetal growth restriction.

Article Snippet: Following this step, primary antibodies including Notch1 (1:750; cat. no. 20687-1-AP), Jagged1 (1:20,000; cat. no. 66890-1-Ig), β-actin (1:20,000; cat. no. 66009-1-Ig), TNF-α (1:2,000; cat. no. 60291-1-Ig), vascular endothelial growth factor (VEGF; (1:8,000; cat. no. 19003-1-AP), GAPDH (1:50,000; cat. no. 60004-1-Ig; all from Proteintech Group, Inc.), IL-6 (1:1,000; cat. no. bs-0782R), placental growth factor (PLGF; 1:1,000; cat. no. bsm-54066R; both from BIOSS), C-X-C motif chemokine ligand 1 (CXCL1; 1:100; cat. no. ab206411), soluble fms-like tyrosine kinase-1 (sFlt-1; 1:1,000; cat. no. ab32152) and placental protein 13 (PP13; 1:1,000; cat. no. ab218411; all from Abcam) were introduced to the membranes for overnight incubation at 4 ̊C.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Immunofluorescence, Immunohistochemistry, Control